4x Tris-Glycine SDS Sample Buffer (25 ml)

Tris-Glycine SDS Sample Buffer (4x) is used to prepare protein samples for denaturing gel electrophoresis using Tris-Glycine gels. It has a pH of 6.8 and contains bromophenol blue as a tracking dye.

To use: Heat the sample in a 1X dilution (reduced or non-reduced) at 85C for 25 minutes for optimal results.

Note: Heating samples at 100C in SDS-containing buffers results in proteolysis.

Composition: 250 mM Tris HCl, 8% SDS, 40% Glycerol, 0.01% Bromophenol blue (Non-reduced). Add -mercaptoethanol (8% V/V) before use (Reduced).

MSDS